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Charge-shift migration on native polyacrylamide gel. Proteins (5 μg) in the presence of 0.5% Triton X-100 or in a mixture of 0.5% Triton X-100–0.25% DOC were separated by native 8% PAGE and stained with Coomassie blue. Lanes: 1 to 3, PBP 2a* in 0.5% CHAPS, 0.5% Triton X-100, and 0.5% Triton X-100–0.25% DOC, respectively; 4, TP domain (star) in 0.5% Triton X-100; 5, TP domain in 0.5% Triton X-100–0.25% DOC; 6, BSA in 0.5% Triton X-100; 7, BSA in 0.5% Triton X-100–0.25% DOC.
