Figure 3.

KRT80 was upregulated after overexpression of HNSCAT1. (a) GSEA was performed based on RNA-seq results after overexpression of HNSCAT1. Notably, cell junction- and adhesion complex-related signaling pathways were significantly upregulated in HNSCAT1-overexpressing cells. (b) A Circos analysis was performed and revealed that KRT80 mRNA expression was elevated. KRT80 is relevant for protein binding, peptide binding, and amide binding and may serve as an important regulator in cell junction and adhesion. (c) A robust correlation between KRT80 and linc01269 (R = 0.52, p < 0.001) was observed in HNSC samples. These data were acquired from GEPIA2 (http://gepia2.cancer-pku.cn), and the data were obtained from TCGA database. (d) RNA-seq revealed that lncHNSCAT1 was upregulated, which resulted in the upregulation of KRT80. (e) Western blotting confirmed that KRT80 was upregulated after HNSCAT1 was overexpressed in SCL-1 and Cal27 cells. GAPDH served as a control.