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. 2022 Aug 4;2022:4156966. doi: 10.1155/2022/4156966

Figure 4.

Figure 4

KRT80 is downregulated in HNSC cells. (a) Pancancer analysis of KRT80 RNA expression in TCGA database. (b) Expression of KRT80 in HNSC and normal samples. These data were acquired from GEPIA2 (http://gepia2.cancer-pku.cn). (c) KRT80 presented lower expression in stage IV HNSC samples than in early-stage HNSC samples. (d, e) Elevated KRT80 expression was associated with favorable outcomes in terms of both (d) disease-free survival (log-rank p = 0.0048) and (e) overall survival (log-rank p = 0.034). (f) Real-time PCR revealed that KRT80 was downregulated in HNSC cell lines. HaCaT cells and primary keratinocytes (PK) served as normal controls. The value of HaCaT cells was set to 1. Experiments were conducted in triplicate, and the results are shown as the mean ± SEM. ∗p < 0.05. (g) Western blotting assays demonstrated that KRT80 protein was upregulated in HNSC cells (lanes 3, 4, and 5) compared to normal control cells (lanes 1 and 2).