Figure 5.

KRT80 serves as a tumor suppressor in HNSC cells. (a) Real-time PCR was conducted to evaluate KRT80 expression after overexpressing KRT80 in SCL-1 and Cal27 cells. (b) Western blotting assays showed that KRT80 protein was increased after overexpression in SCL-1 and Cal27 cells. (c, d) Colony formation assays were conducted to evaluate proliferation capacity after KRT80 overexpression in SCL-1 and Cal27 cells. Experiments were conducted in triplicate, and the results are shown as the mean ± SEM. ^∗p < 0.05. (e, f) Transwell assays showed that migration was impaired in KRT80-overexpressing SCL-1 and Cal27 cells. Experiments were conducted in triplicate, and the results are shown as the mean ± SEM. ^∗p < 0.05. (g, h) Subcutaneous xenografts were established in (g) KRT80-overexpressing and control cells. N = 5 for each group. (h) Tumor weight in each xenograft. ^∗p < 0.05.