Skip to main content
. 2022 Aug 4;2022:4156966. doi: 10.1155/2022/4156966

Figure 7.

Figure 7

miR-1254 promotes HNSC malignant behavior by inhibiting KRT80. (a, b) Colony formation assays were performed to determine proliferation capacity after overexpressing miRNA-1254 in SCL-1 and Cal27 cells. Experiments were conducted in triplicate, and the results are shown as the mean ± SEM. ∗p < 0.05. (c, d) Transwell assays showed that the migration ability was impaired after overexpressing miRNA-1254 in SCL-1 and Cal27 cells. All of the experiments were performed in triplicate and are presented as the mean ± SEM. ∗p < 0.05. (e, f) The CCK-8 assay indicated that the cell growth inhibition effect after HNSCAT1 overexpression was largely rescued when miR-1254 was reintroduced; however, the rescue efficacy was diminished after the addition of extra miR-1254 inhibitors in both (e) SCL-1 and (f) Cal27 cells. (g) Proposed mechanism of the lncRNA HNSCAT1/miR-1254/KRT80 axis in HNSC. HNSCAT1 could interact with miR-1254 and thereby prevent miR-1254-mediated KRT80 degradation. lncHNSCAT1 and KRT80 serve as important tumor suppressors in HNSC.