Figure 2.

Electroacupuncture of Weizhong (BL-40) acupoints significantly promotes myoblast determination protein (MYOD) of back muscle in young and aged rats. (a) Representative images of MYOD immunohistochemically staining of the back muscles of rats in the following groups, YS: young sham group (n = 8); YI: young injured group (n = 7); YI + WZ: young injured rats treated with electroacupuncture of Weizhong (BL-40) acupoint (n = 7); YI + NWZ: young injured rats treated with electroacupuncture of non-Weizhong (BL-40) acupoint (n = 7); AS: aged sham group (n = 8); AI: aged injured rats (n = 7); AI + WZ: aged injured rats treated with electroacupuncture of Weizhong (BL-40) acupoint (n = 7); AI + NWZ: aged injured rats treated with electroacupuncture of non-Weizhong (BL-40) acupoint (n = 7); The electroacupuncture last for 10 days after the rat back muscle injured for 5 days. Scale bar, 100 μm. The red arrow in the figures points to MYOD-positive cells. (b) Quantification analysis of average MYOD positive cell number of all rats (n = 8 in young sham and aged sham groups, n = 7 in other groups). All data are represented as the Mean ± SD. Student's t-test was used for all statistical analyses (^ϯϯϯP < 0.001,^#P < 0.05,^##P < 0.01).