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SDS-PAGE analysis of MnCCD proteins expressed in E. coli BL21 (DE3) cells and their purification. M: 180 kDa protein marker. (a) Lane 1: control, lanes 2–7: Mn008741, MnCCD1B1, MnCCD1A, MnCCD4, MnCCD1B2, and Mn008739; (b) lanes 1–6: the purified MnCCD proteins. Target protein bands are marked with black boxes, and the tagged protein band is marked with a red box.
