. 2022 Aug 4;2022:4811144. doi: 10.1155/2022/4811144

Table 1.

Primers used in this study.

Gene	Primer (5′-3′)	Used for
MnCCD1A	ATGGCGGAAGTGGCGTC	RT-PCR
MnCCD1A	GATATTCAGTTTGAGTCCGAGTA	RT-PCR

MnCCD1A	atggagctcggtaccATGGCGGAAGTGGCGTC	Prokaryotic expression vector construction
MnCCD1A	caggtcgacaagcttgaattcGATATTCAGTTTGAGTCCGAGTA	Prokaryotic expression vector construction

MnCCD1B1	ATGGCCGAAATAGTGGATGTGAAT	RT-PCR
MnCCD1B1	TAGGACACCATTCCCAACATCAA	RT-PCR

MnCCD1B1	atggagctcggtaccATGGCCGAAATAGTGGATGTGAAT	Prokaryotic expression vector construction
MnCCD1B1	caggtcgacaagcttgaattcTAGGACACCATTCCCAACATCAA	Prokaryotic expression vector construction

MnCCD1B2	GTGGGACTGTTCAAGGC	RT-PCR
MnCCD1B2	CTCTGCTGGGCAAATC	RT-PCR

MnCCD1B2	atggagctcggtaccATGGCGGAGAAGCCGAGC	Prokaryotic expression vector construction
MnCCD1B2	caggtcgacaagcttgaattcGAGTTTTGCTTGTTCTTGCAGTTG	Prokaryotic expression vector construction

MnCCD4	ATGGCTTCCTCTTTTTTGGCAC	RT-PCR
MnCCD4	TCATGCCTGGTGAACCAAGTCC	RT-PCR

MnCCD4	atggagctcggtaccATGGCTTCCTCTTTTTTGGCAC	Prokaryotic expression vector construction
MnCCD4	caggtcgacaagcttgaattcTCATGCCTGGTGAACCAAGTCC	Prokaryotic expression vector construction

Mn008739	ATGCCACATCACGTCTCCAA	RT-PCR
Mn008739	CACCGAGACTGGTATGAAAGCT	RT-PCR

Mn008739	atggagctcggtaccATGCCACATCACGTCTCCAA	Prokaryotic expression vector construction
Mn008739	caggtcgacaagcttgaattcCACCGAGACTGGTATGAAAGCT	Prokaryotic expression vector construction

Mn008741	ATGGCATCATCGTATATGGCAT	RT-PCR
Mn008741	TGGTGAGATTGGTATGAAAGCT	RT-PCR

Mn008741	atggagctcggtaccATGGCATCATCGTATATGGCAT	Prokaryotic expression vector construction
Mn008741	caggtcgacaagcttgaattcTGGTGAGATTGGTATGAAAGCT	Prokaryotic expression vector construction

Lowercase letters represent the homology arms containing Kpn I or ECOR I restriction sites.