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. 2022 Jul 25;50(14):8240–8261. doi: 10.1093/nar/gkac631

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Free eIF4A with bound ATP stimulates eIF4E–mRNA association independently of eIF4G. (A) Representative single-molecule trace showing eIF4E–mRNA interaction in the presence of 2 μM eIF4A and 2.5 mM ATP. (B) eIF4E-mRNA association rates in the presence (red) and absence (grey) of eIF4A and ATP, compared with eIF4E-only rates. (C) eIF4E–mRNA association rates in the presence of eIF4A and ATP or ATP-γ-S, for the NCE102 and JJJ1 mRNAs. (D) eIF4E–mRNA dissociation rates in the presence of free eIF4A and ATP. (E) Relationship between translation-efficiency dependence on eIF4A and the fold-increase in eIF4E–mRNA association rate induced by free eIF4A and ATP.