FIG. 4.

Physical analysis of Em^r transformants carrying the insertion of pVA891 within ORF13 in Tn5252. Autoradiogram showing Southern hybridization of ³²P-labeled pDR6 to ClaI- (A) and HindIII- (B) digested chromosomal DNA from SP1291 (SP1000 carrying a deletion within ORF13) (lane 1), SP1000 (Rx1::Tn5252) (lane 2), and S. pneumoniae Rx1 (lane 3). The indicated sizes correspond to the standards in lane M, which consist of a set of calibrated fragments from pSK(+) or derivative plasmids, all of which react with the probe.