TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| S. pneumoniae | ||
| Rx1 | Nonencapsulated laboratory strain | 1 |
| DP1110 | UVs | W. R. Guild |
| SP1000 | str-1 fus Tn5252 | 1 |
| SP1254 | str-1 Tn5252ΩEm Tra+ | 10 |
| SP1291 | str-1 Tn5252ΩEm | This study |
| SP1311 | str-1 derivative of DP1110 | This study |
| SP1317 | SP1311(pSJ142 Spcr) | This study |
| SP1323 | SP1311(pAT29) | This study |
| SP1324 | SP1311(pSJ142Δ0.5-kb XbaI fragment) | This study |
| SP1402 | SP1311::Tn5252 | This study |
| SP1405 | SP1311::Tn5252ΩEm | This study |
| S. pyogenes | ||
| ATCC 19615 | opt | Stillwater Medical Center |
| E. faecalis | ||
| JH2-2 | Wild type, plasmid free | 32 |
| UV202 | fus rif UVs | 32 |
| SF5002 | UV202::Tn5252 | 1 |
| SF5004 | UV202(pSJ142, Spcr) | This study |
| E. coli | ||
| JM109 | F′ lacIq Δ(lacZ)M15 proA+B+/Δ(lac-proAB) recA1 | 33 |
| AB1157 | his-4 | 11 |
| RM1140 | Same as AB1157 but umuC36 | R. V. Miller |
| Plasmids | ||
| pAT29 (6.7 kb) | lacZ Spcr | 29 |
| pDR6 | KS(+)::4.5-kb DNA fragment (uvr operon of Tn5252) | This study |
| pKM101 | R46 derivative carrying the mucAB operon | 17 |
| pSJ142 | pAT29::4.5-kb fragment DNA (uvr operon of Tn5252) | This study |
| pSE117 | pBR322 derivative::E. coli umuCD operon | 15 |
| pVA891 (5.9 kb) | Cm Em | 14 |