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. 2022 Jul 8;50(14):8143–8153. doi: 10.1093/nar/gkac580

Figure 4.

Figure 4.

Effect of RGB-1 on translation from the 5′UTR of Nectin-4 mRNA. (A) The mRNAs encoding firefly luciferase (FL) with a native 5′UTR of Nectin-4 and mutated versions of its 5′UTR were designed as models. Renilla luciferase (RL) was placed downstream of an internal ribosomal entry site (IRES) as an internal expression control. (B)The nucleotide sequences of the 5′UTRs of the model mRNAs. Luc indicates the firefly luciferase gene. The G-tracts were shown in red. The A-tracts, which were replaced from G-tracts, are shown in blue. (C) Effect of RGB-1 on translation of reporter RNA in vitro. RGB-1 showed no detectable effect on translation from the non-structured Nectin-4 mutant1 RNA template, but, as expected, reduced translation from Nectin-4 wild-type and mutant7 RNA templates that contain G-quadruplex structures. Data are shown as mean ± SD. Statistical significance was determined by Student's t-test: n.s. = no statistical significance, **P < 0.01 and ***P < 0.001, compared with DMSO alone.