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. 2022 Jul 12;50(14):7906–7924. doi: 10.1093/nar/gkac600

Figure 2.

Figure 2.

NURF301 promotes gypsy insulator barrier activity. (A) Schematic diagram of in vivo insulator barrier assay. In the non-insulated UAS-luciferase line, spreading of repressive chromatin leads to low expression of luciferase. In the insulated line, presence of the gypsy insulator acts as a barrier and allows for high expression of luciferase. (B) Western blotting of male second instar larval extracts for NURF301, CP190, and Tubulin (loading control) in control flies and NURF301RNAi knockdown flies using the Act5C-Gal4 driver grown at 25°C. (C, D). Relative luciferase activity of insulated or non-insulated male (C) and female (D) third instar larvae of control or with NURF301RNAi driven by Mef2-Gal4 driver. Su(Hw)RNAi is used as a positive control. For each genotype, n = 12 individual larvae, with each experiment performed twice with similar results. One-way ANOVA followed by Tukey HSD post hoc test was used to calculate P-values for pairwise comparisons. Box represents the 25–75th percentiles, and the median is indicated. The whiskers show minimum and maximum values. P-values are indicated.