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. 2022 Jul 8;50(14):8008–8022. doi: 10.1093/nar/gkac583

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — SMARCAL1 and ZRANB3 physically interact with RAD51 and BCDX2. (A) Soluble extract from E. coli containing MBP-RAD51 (bait) was immobilized on amylose resin and incubated with purified recombinant proteins (RAD51 paralogs BCDX2, ZRANB3 or SMARCAL1, [prey]) as indicated. Western blot analyses were performed with anti-MBP and anti-FLAG antibodies. (B) and (C) Anti-His antibody was coupled to Protein G agarose, bound to the BCDX2 complex (bait) and tested for interaction with ZRANB3 (prey) or SMARCAL1 (prey), respectively. Samples were subjected to either silver staining or Western blot analysis with anti-FLAG and anti-His antibodies.