Table 1.
Cryo-EM collection and processing statistics
| Data collection and processing | Nsp15/dsRNA dataset 1 (EMD-26073, PDB: 7TQV) | Nsp15/dsRNA dataset 2 (EMD-25915 PDB: 7TJ2) |
|---|---|---|
| Microscope | Talos Arctica | Titan Krios |
| Detector | Gatan K2 Summit | Gatan K3 Bioquantum |
| Nominal magnification | 45 000× | 81 000× |
| Voltage (kV) | 200 | 300 |
| Electron exposure (e–/Å2) | 54 | 60 |
| Defocus range (μm) | −0.8 to -1.8 | −1.2 to 2.2 |
| Pixel size (Å) | 0.93 | 0.53 |
| Symmetry imposed | C1 | C1 |
| Number of Micrographs | 2435 | 752 |
| Initial particle images | 1 201 304 | 254 136 |
| Final particle images | 398 516 | 97 592 |
| Refinement | Dataset 1 | Combined Datasets |
| Resolution (Å)/FSC threshold | 3.43/0.143 | 3.19/0.143 |
| B-factor used for map sharpening (Å2) | 162.7 | 171.3 |
| Map to model CC | ||
| CC (mask) | 0.69 | 0.76 |
| CC (volume) | 0.68 | 0.74 |
| CC (peaks) | 0.56 | 0.65 |
| CC (box) | 0.68 | 0.69 |
| Model composition | ||
| Non-hydrogen atoms | 17 793 | 17 084 |
| Protein residues | 2083 | 2007 |
| Nucleic acid | 66 | 62 |
| Mean B factors (Å2) | ||
| Protein | 85.95 | 58.77 |
| Nucleic acid | 277.22 | 150.81 |
| R.m.s. deviations | ||
| Bond lengths (Å) | 0.002 | 0.002 |
| Bond angles (°) | 0.383 | 0.370 |
| Validation | ||
| Molprobity score | 1.52 | 1.39 |
| Clashscore | 5.00 | 3.74 |
| Poor rotamers (%) | 0.82 | 1.64 |
| Ramachandran plot | ||
| Favored (%) | 96.18 | 97.69 |
| Allowed (%) | 3.82 | 2.31 |
| Disallowed (%) | 0.00 | 0.00 |