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. 2022 Aug 11;13:4709. doi: 10.1038/s41467-022-32138-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Volcano plot of differential expressed protein-coding genes (Fold Change greater than 2 and FDR less than 0.05). b, c Enriched GO term (b) and KEGG pathway (c) analysis of downregulated genes in TCKO cells. d Barplot of genome-wide CG methylation level in TCKO and WT cells. e Metaplot of CG methylation level over protein-coding genes in TCKO and WT cells. f Pie chart of hypermethylated and hypomethylated CG-DMR counts in TCKO and WT cells. TSS transcription start site, TTS transcription termination site.