Table 1.
Summary of jet speeds, experimental conditions, and statistics for lysozyme crystals1
| Target jet speed | 50 m/s | 75 m/s | 110 m/s* |
|---|---|---|---|
| Liquid flow (µl/min) | 15 | 13 | 13 |
| Gas flow (mg/min) | 23 | 50 | 85 |
| Experimental jet speed (m/s) | 42 ± 2.1 | 78 ± 3.9 | – |
| Theoretical jet speed (m/s) | – | – | 102 ± 5.1 |
| Total no. frames | 440,000 | 60,000 | 240,000 |
| No. of hits | 10,726 (2.4%) | 1,638 (2.7%) | 3,733 (1.6%) |
| No. of indexed frames | 9,970 (93%) | 1,509 (92.1%) | 3,474 (93.1%) |
| No. double hits | 1190 | 28 | 20 |
| No. single hits | 8780 | 1481 | 3454 |
This table shows the flow rates of the gas and liquid used as well the experimentally and theoretically determined jet speeds for the current analysis. The data statistics were calculated utilising the CrystFEL software suite44,45.
* A 100 m/s jet speed was determined experimentally for a 13 µl/min liquid and 80 mg/min gas flow rate giving a speed of 105 m/s, which is similar to the theoretically calculated 102 m/s jet speed.