Fig. 2. CircTmem241 drives ILC3 differentiation from the ILCP progenitor stage.

a–c FACS analysis of circTmem241^+/+ and circTmem241^−/− ILC lineage progenitors. Plots displayed frequencies of CLPs (Lin⁻CD127⁺c-Kit^intSca-1^int gated), α₄β₇⁺CLPs (Lin⁻CD127⁺c-Kit^intSca-1^int gated), CHILPs (Lin⁻CD25⁻CD127⁺Flt3⁻ gated) and ILCPs (Lin⁻CD127⁺Flt3⁻c-Kit⁺ gated). n = 5 for each group. ns, no significance. d Numbers of indicated progenitor cells in a–c were calculated. n = 5 for each group. ns, no significance. e–g Precursor cells ILC1P (Lin⁻CD127⁺Eomes⁻CD49a⁺ gated), ILC2P (Lin⁻CD127⁺CD45⁺Flt3⁻CD117⁻Sca-1⁺ gated) and ILC3P (Lin⁻CD25⁻CD127⁺α4β7^intGata3^lowBcl11b⁺ gated) were analyzed in circTmem241^+/+ and circTmem241^−/− mice by FACS. n = 5 for each group. h Numbers of indicated ILC precursor cells in e–g were calculated. n = 5 for each group. ns, no significance. i–j CHILPs (Lin⁻CD25⁻CD127⁺Flt3⁻α4β7⁺) or ILCPs (Lin⁻CD25⁻CD127⁺Flt3⁻α4β7⁺c-Kit⁺PD-1⁺) were sorted from circTmem241^+/+ and circTmem241^−/− mice and cultured under in vitro differentiation condition for 7 or 14 days. Flow cytometric analysis of indicated cells was performed and absolute numbers were calculated. n = 5 for each group. **P < 0.01 and ***P < 0.001. Data were analyzed by an unpaired two-side Student’s t test and shown as means ± SD. Data are representative of at least three independent experiments. Source data are provided as a Source Data file.