Fig. 3. CircTmem241 regulates ILC3 commitment in an intrinsic manner.

a Schematic diagram of bone marrow transplantation. b 5 × 10⁶ CD45.2⁺ BM cells from circTmem241^+/+ or circTmem241^−/− mice were transferred into lethally irradiated CD45.1⁺ recipients. 8 weeks later, ILC3s (Lin⁻CD45.2⁺CD127⁺RORγt⁺) were analyzed. n = 5 for each group. c 1:1 mixture of CD45.1⁺ wild-type and CD45.2⁺ circTmem241^+/+ or circTmem241^−/− BM cells were transferred into lethally irradiated CD45.1⁺ recipient mice for 8 weeks. Then ratios of CD45.1⁺ to CD45.2⁺ ILC3s in chimeras were analyzed by gating on CD45.2⁺Lin⁻RORγt⁺ (circTmem241^+/+ or circTmem241^−/−) and CD45.1⁺Lin⁻RORγt⁺ (WT). n = 5 for each group. d Irradiated recipient mice were reconstructed with circTmem241^+/+ or circTmem241^−/− BM cells as indicated in a (left). Colons isolated from reconstructed mice infected with C. rodentium were subjected to HE staining. Scale bars, 100 μm. e Histological scores of colons from reconstructed mice in d. n = 5 for each group. f Reconstituted mice, as indicated in d, were infected with C. rodentium and survival rates were measured. N = 10 for each group. **P < 0.01 and ***P < 0.001. Data were analyzed by an unpaired two-side Student’s t test and shown as means ± SD. Data are representative of at least three independent experiments. Source data are provided as a Source Data file.