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. 2022 Aug 12;55(10):1924–1939.e5. doi: 10.1016/j.immuni.2022.08.003

Figure 5.

Figure 5

T cell reactivity against SARS-CoV-2 Omicron varies between individuals

(A) Representative dot plot examples showing antigen-reactive T cells against the full-length spike, a pool of 80 peptides affected by the Omicron B.1.1.529 BA.1 mutations (Omicron peptides), and the corresponding wild-type peptides from one donor. Absolute cell counts after magnetic CD154+ enrichment from 1x10e7 PBMCs are indicated.

(B) Frequencies of reactive CD4+ memory T cells against the indicated antigens analyzed post-third vaccination (n = 45).

(C) Proportion of CD45RA− memory T cells within reactive CD154+CD4+ T cells (n = 45) and representative CD45RA and CCR7 staining for the wild-type and Omicron peptide-reactive T cells.

(D) Frequencies of reactive CD4+ Tmems against the wild-type and Omicron peptides analyzed post-third vaccination in different age groups (<25, n = 9; 26–39, n = 17; 40–60, n = 9; >80, n = 7).

(E) Donors were grouped by their pre-vaccination frequencies of spike-reactive Tmems as described in Figure 3, and the percentage of total reduction in spike-specific T cells by the Omicron peptides is depicted. Each symbol in (B–E) represents one donor; horizontal lines indicate mean in (B, C, E) and geometric mean in (D). Statistical differences: Kruskal-Wallis test with Dunn’s post hoc test in (B–D).