Table 1.

Survey of garlic plants in Tokachi garlic-propagation fields to detect LYSV-N and LYSV-S using PCR

Year	No. of garlic plants infected with LYSV with N- and S-type P1
	LYSV-positive (total tested)	N-type	S-type	N + S
2019	23 (40)	20	0	3
2020	20 (60)	20	0	0

Primer pair LY5P/LY2M was used for the first-round PCR, S-263F/S-696R for nested-PCR. New virus-free seedlings are introduced to regions every year.

LYSV, leek yellow stripe virus; PCR, polymerase chain reaction.