Table 1.
List of strains and plasmids used in this study.
| Strain/plasmid | Genotype and description | Source |
|---|---|---|
| S. cerevisiae | ||
| BTCC3 | Wild type, haploid | InaCC |
| BTCC3 LX1 |
∆PDC1::PTDH3–LcLLDH–TTDH3 BTCC3 harboring pAUR101-TDH3pro-LcLLDH-dPDC1 |
This study |
| BTCC3 LX5 |
∆PDC5:: PTDH3–LcLLDH–TTDH3 BTCC3 harboring pAUR101-TDH3pro-LcLLDH-dPDC5 |
This study |
| BTCC3 LA1 |
∆PDC1::PTDH3–LcLLDH–TTDH3 ∆PDC5 BTCC3 harboring pAUR101-TDH3pro-LcLLDH-dPDC1 and pPC01-BTCC3PDC5KO |
This study |
| BTCC3 LA15 |
∆PDC1::PTDH3–LcLLDH–TTDH3 ∆PDC5::PTDH3–LcLLDH–TTDH3 BTCC3 harboring pAUR101-TDH3pro-LcLLDH-dPDC1 and pAUR101-TDH3pro-LcLLDH-dPDC5 |
This study |
| BTCC3 LA2 |
∆PDC1::LcLLDH ∆PDC5 BTCC3 harboring pAUR101-BTCC3PDC1-LcLLDH and pPC01-BTCC3PDC5KO |
This study |
| Plasmids | ||
| pAUR101-BTCC3PDC1-LcLLDH | PPDC1–LcLLDH–TPDC1, AMPR, AUR1-C | This study |
| pAUR101-TDH3pro-LcLLDH-dPDC1 | PDC1, PTDH3–LcLLDH–TTDH3, AMPR, AUR1-C | This study |
| pPC01-BTCC3PDC5KO | PDC5, KANR, KanMx | This study |
| pAUR101-TDH3pro-LcLLDH-dPDC5 | PDC5, PTDH3–LcLLDH–TTDH3, AMPR, AUR1-C | This study |