Fig. 4. Acute and chronic hypothalamic and prefrontal cortex (PFC) transcriptomes following a single high dose of psilocybin.

A Schematic overview of bulk RNA-seq study. Lean mice were split into two groups and received either a single dose of psilocybin (3 mg/kg) or a vehicle (n = 16 per group). Three hours following dosing, eight psilocybin-treated and eight vehicle-treated mice were sacrificed, and PFC and whole hypothalamus were dissected. The remaining mice were sacrificed 4 weeks after injection. B Schematic showing the effect of psilocybin in the PFC 3 h after administration. C Top 10 significantly differentially expressed genes in PFC (3 h after administration of psilocybin). Points in pink represent genes with p.adj < 0.05. D Reactome pathways for upregulated and downregulated genes in PFC 3 h after administration of psilocybin. E Volcano plot showing the effect of psilocybin in the hypothalamus 3 h after administration of psilocybin vs. vehicle. F Volcano plot showing the effect of psilocybin in PFC 4 weeks after administration of psilocybin vs. vehicle. G Volcano plot showing effect in the hypothalamus for psilocybin-treated versus vehicle-treated mice 4 weeks after administration. Cut off for volcano plots B, E, F, and G calculated as 0.05/19080 number of tested genes.