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. 2022 Jul 14;47(10):1755–1763. doi: 10.1038/s41386-022-01376-4

Fig. 3. Prenatal opioid exposure decreases microglial engulfment of NAc-D1R during adolescence in males.

Fig. 3

A Schematic of timeline for exposure and P30 engulfment assessment. B D1R volume within microglia was significantly decreased following oxycodone exposure in male offspring at P30. C % D1R volume of total microglial volume was significantly decreased following oxycodone exposure in male offspring at P30. D There was no treatment effect on microglial volume. E Representative 60x images of microglial D1R engulment: grey: Iba1, green: D1R, scale bar = 5 microns. F Scholl analysis revealed no differences in # of intersections between control and oxycodone males, or in branch endpoints (G) or Ramification Index (H). B–H Dots represent individual microglia, N = 29 (control), 30 (oxycodone) nested per animal 6 and 7, respectively for analysis (nested t-tests). Data represent Mean +/− SEM, *p = 0.05.