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Effects of artificial electron donors and azide on the activity of nitrate reduction in wild-type strain G-179a
| Assay | Sp act (nmol of nitrate min−1 mg of protein−1 [±SD]) with indicated electron donor
|
|||
|---|---|---|---|---|
| MV+ | BV+ | BV+ + azide | NADH | |
| Whole cell | 337 ± 15 | 395 ± 7 | 401 ± 34 | ND |
| Crude extract | 277 ± 14 | 310 ± 20 | 303 ± 20 | nd |
Cells were grown under anaerobic conditions with nitrate as the electron acceptor. When used the concentration of azide was 40 μM. ND, not determined; nd, not detected.
