Figure 1.

Cell sorting and the CD133 positivity after cell culture of CD133-positive subsets in HepG2 and Hep3B hepatoma cell lines. (A) The CD133-positive subsets in HepG2 and Hep3B cell lines were sorted by flow cytometry, the proportion of the subsets were 19.8%, 72.4%, respectively. About 60% of CD133⁺ cells presenting strongly positive were sorted. The sorted cells were cultured with DMEM/F12, EGF, bFGF, and B27. (B) The CD133 positivity of CD133⁺HepG2 and CD133⁺Hep3B cell lines which detected by flow cytometry were 74%, 90.5%, respectively. EGF, recombinant epidermal growth factor; bFGF, basic fibroblast growth factor.