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. 2022 Jul;11(7):2013–2025. doi: 10.21037/tcr-22-298

Figure 2.

Figure 2

Knockdown of LOXL2 impairs the spheroid formation of LCSCs. CD133+ (A) HepG2 and (B) Hep3B cells for sphere-forming assay were transfected with shRNA LOXL2-LV or NC-LV to observe cell survival upon LOXL2 knockdown. After culturing for 10–15 days, the number of tumor spheroids (>50 µm) was counted. LOXL2 knockdown impaired the cell sphere-forming, especially the CD133+Hep3B cells presenting with growth retardation. CD133+Hep3B cells in the following study were shifted to transiently transfection with LOXL2-siRNA or NC-siRNA. (C) Western blotting and (D) PCR were performed to determine transfection efficiency of the two ways. Relative intensity values for the proteins were obtained using Image J software. The data were analyzed upon three independent experiments and shown as mean ± SD. **, P<0.01; ***, P<0.001; ****, P<0.0001. NC-LV, lentiviral vector against negative control; LOXL2-LV, lentiviral vector against LOXL2; LOXL2, lysyl oxidase-like 2; LOXL2-siRNA, siRNAs against LOXL2; NC-siRNA, siRNAs against negative control; LCSCs, liver cancer stem cells; SD, standard deviation.