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. 2022 Jul;11(7):2013–2025. doi: 10.21037/tcr-22-298

Figure 3.

Figure 3

Knockdown of LOXL2 inhibits LCSCs migration and invasion. The transwell (A) migration and (B) invasion assays of CD133+HepG2 cells were performed upon LOXL2 knockdown by transfected with shRNA lentiviral vector (LOXL2-LV/NC-LV). After culturing for 24 hours in the transwells, cells were fixed and stained by 0.1% crystal violet. The average number of migrating and invading cells are showed in graphs. The transwell (C) migration and (D) invasion assays of CD133+Hep3B cells were performed upon LOXL2 knockdown by transfected with siRNAs (LOXL2-siRNA/NC-siRNA). Scale bar: 50 µm. The data were analyzed upon three independent experiments and shown as mean ± SD. *, P<0.05; **, P<0.01. NC-LV, lentiviral vector against negative control; LOXL2-LV, lentiviral vector against LOXL2; LOXL2, lysyl oxidase-like 2; LOXL2-siRNA, siRNAs against LOXL2; NC-siRNA, siRNAs against negative control; LCSCs, liver cancer stem cells; SD, standard deviation.