Skip to main content
. 2022 Jul;11(7):2013–2025. doi: 10.21037/tcr-22-298

Figure 4.

Figure 4

Knockdown of LOXL2 induces apoptosis and cell cycle arrest in LCSCs. CD133+HepG2 and CD133+Hep3B cells for flow cytometry analysis were transfected with shRNA lentiviral vector (LOXL2-LV/NC-LV) or siRNAs (LOXL2-siRNA/NC-siRNA), respectively, to knockdown LOXL2. Cells for apoptosis were gated by Annexin V-FITC and PI staining. Knockdown of LOXL2 induced the apoptosis of CD133+ (A) HepG2 and (B) Hep3B cells. LOXL2 knockdown also induced cell cycle arrested in CD133+ (C) HepG2 and (D) Hep3B cells. Data represented as % of total cells. The data were analyzed upon three independent experiments and presented as mean ± SD. *, P<0.05; **, P<0.01. NC-LV, lentiviral vector against negative control; LOXL2-LV, lentiviral vector against LOXL2; LOXL2, lysyl oxidase-like 2; LOXL2-siRNA, siRNAs against LOXL2; NC-siRNA, siRNAs against negative control; LCSCs, liver cancer stem cells; SD, standard deviation.