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. 2022 Jul;11(7):2013–2025. doi: 10.21037/tcr-22-298

Figure 6.

Figure 6

Knockdown of LOXL2 inhibits the expression of autophagy marker LC3B and autophagy gene ATG5 in LCSCs. Western blotting and PCR of CD133+ (A,B) HepG2 and (C,D) Hep3B cells were performed upon LOXL2 knockdown to determine the expression of LC3B and ATG5 by transfected with shRNA lentiviral vector (LOXL2-LV/NC-LV) or siRNAs (LOXL2-siRNA/NC-siRNA) respectively. Relative intensity values for the proteins were obtained using Image J software. (E,F) Immunofluorescence staining was performed for LC3B and ATG5 in CD133+HepG2 cells upon LOXL2 knockdown. Scale bars 200 µM. Mean fluorescence intensity values were obtained using Image J software. The data were analyzed upon three independent experiments. *, P<0.05; **, P<0.01. LC3B, microtubule-associated protein 1 light chain 3 B; NC-LV, lentiviral vector against negative control; LOXL2-LV, lentiviral vector against LOXL2; LOXL2, lysyl oxidase-like 2; LOXL2-siRNA, siRNAs against LOXL2; NC-siRNA, siRNAs against negative control; LCSCs, liver cancer stem cells.