TABLE 1.
Plasmids used in this study
| Plasmid | Resistance determinantsa | Replicon | Size (kb) | Key genetic properties | Reference |
|---|---|---|---|---|---|
| pCT800 | Pnr | pMB1 | 7.2 | tacp-His6incC1 | This work |
| pDM1.1 | Smr | IncQ | 11.6 | tacp | 21 |
| pDM1.12 | Smr Tcr | IncQ | 13.0 | tacp-korB | 21 |
| pDM3.1 | Kmr | pSC101 | 10.4 | korAp-xylE | 21 |
| pDM300 | Kmr | pSC101 | 10.2 | kfrAp-xylE | 21 |
| pGBT30 | Pnr | pMB1 | 6.0 | tacp | 16 |
| pGBT36 | Pnr | pMB1 | 7.1 | tacp-incC1 | This work |
| pGBT37 | Pnr | pMB1 | 6.4 | tacp-korA | 12 |
| pGBT58 | Kmr | pSC101 | 10.2 | trfAp-xylE | 12 |
| pGBT59 | Kmr | pSC101 | 10.2 | trbAp-xylE | 13 |
| pGBT63 | Kmr | pSC101 | 10.5 | trbBp-xylE | 12 |
| pGBT70 | Kmr | pSC101 | 10.2 | trfAp-1-xylE | 15 |
| pGBT71 | Kmr | pSC101 | 10.2 | trbAp-xylE | 15 |
| pGBT302 | Pnr | pMB1 | 6.8 | tacp-incC2 | This work |
| pGBT400 | Smr | IncQ | 11.6 | tacp | This work |
| pGBT401 | Smr | IncQ | 11.9 | tacp-korA | This work |
| pKK1 | Tcr | IncP | 9.6 | trfAp-1 | 19 |
| pOLE1 | Smr | IncQ | 12.7 | tacp-incC1 | This work |
a
Pnr, Kmr, Smr, and Tcr, resistance to penicillin, kanamycin, streptomycin, and tetracycline, respectively.