TABLE 2.
Loss of RK2 from E. coli C600K in response to the expression of incCa
| lacIqtacp plasmid (no. of generations) | CFU on L agar + Sm + Km (pDM1.1 or pOLE1 plus RK2)/CFU on L agar + Sm (pDM1.1 or pOLE1) with:
|
|||
|---|---|---|---|---|
| 0 mM IPTG | 0.02 mM IPTG | 0.1 mM IPTG | 0.5 mM IPTG | |
| pDM1.1 (0) | 1.0 | 1.0 | 1.0 | 1.0 |
| pDM1.1 (20) | 0.88 | 0.95 | 0.93 | 1.16 |
| pOLE1 (0) | 0.95 | 0.95 | 0.95 | 0.95 |
| pOLE1 (20) | 0.62 | 0.73 | 2.9 × 10−2 | 8.7 × 10−6 |
Sm, streptomycin; Km, kanamycin.