TABLE 3.
Effect of overexpression of incC on repression by korB
| Promoter and KorB operator | xylE reporter plasmid | Locationa | XylE activity (standard units)b for:
|
||
|---|---|---|---|---|---|
| pDM1.1/pGBT30 (−/−) | pDM1.12/pGBT30 (korB/−) | pDM1.12/pGBT36 (korB/incC) | |||
| korAp OB1 | pDM 3.1 | p/u | 0.85 | 0.3 | 0.15 |
| trfAp OB10 | pGBT58 | p/u | 3.12 | 0.25 | 0.1 |
| trfAp-1 OB10 | pGBT70 | p/u | 0.26 | 0.10 | 0.03 |
| trbAp* OB10 | pGBT71 | d/d | 0.09 | <0.01 | <0.01 |
| trbBp OB9 | pGBT63 | d/u | 1.50 | 0.25 | 0.05 |
| kfrAp OB2 | pDM300 | d/u | 0.60 | 0.15 | 0.05 |
a
Location is indicated as proximal (p [close to]) or distal (d [well removed from]) and upstream (u) or downstream (d).
b
Activities are representative results from at least three experiments. The effect of pGBT36 (tacp-incC) in the presence of pDM1.1 (tacp control) showed no repression on any of the promoters. The level of IPTG used in this experiment to induce repressor expression was 0.5 mM.