Figure 1.

The kidney displays high abundance of T_RM cells

See also Figure S1. (A) Unsupervised cell cluster detection and quantification by t-distributed stochastic neighbor embedding (TSNE) and cytometry by time-of-flight (CyTOF) cluster detection algorithm on renal CD45⁺ cells from normal human kidneys (n = 6). (B) Unsupervised cell cluster detection and quantification by TSNE and CyTOF cluster detection algorithm on renal CD45⁺ cells from normal mouse kidneys (n = 6). (C) Gating strategy for renal intravascular staining after intravenous (i.v.) injection of allophycocyanin (APC) conjugated CD45 antibody. (D) The median fluorescence intensity (MFI) of CXCR3 and CD62L in renal CD69⁻CD44⁺ and CD69⁺CD44⁺ T cells individually (n = 8). (E) The MFI of IFN-γ and perforin in renal CD69⁻CD44⁺ and CD69⁺CD44⁺ T cells individually (n = 8). Data are represented as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01.