Figure 2.

The proportion of CD8⁺ T_RM cells is significantly increased in mice and patients with glomerular diseases

See also Figure S2. (A–C) Representative flow cytometric analyses and quantification of renal CD8⁺ T_RM (CD8⁺CD69⁺CD44⁺) cells in ADR-treated mice (A), db/db mice (B), MRL/lpr mice, (C) and their controls (n = 8). (D) The frequency of IFN-γ and perforin in renal CD8⁺ T_RM cells in control and ADR-treated mice (n = 8). (E) The frequency of IFN-γ (n = 8) and perforin (n = 6) in renal CD8⁺ T_RM cells in db/m and db/db mice. (F) The frequency of IFN-γ and perforin in renal CD8⁺ T_RM cells in control and MRL/lpr mice (n = 6). (G) Representative immunofluorescence staining for CD8 (green), CD69 (red), and CD45RO (gray) in human renal tissues from normal, subjects with FSGS diabetic nephropathy (DN) and LN. Left: representative images, white arrows highlight CD8⁺ T_RM cells (CD8⁺CD69⁺CD45RO⁺); scale bars: white, 70 μm; red 5, μm; right: quantification of CD8⁺ T_RM cells surrounding the glomeruli per high power field (HPF, n = 8). Data are represented as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ns, no significance.