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. 2022 Apr 27;30(8):2709–2721. doi: 10.1016/j.ymthe.2022.04.019

Figure 4.

Figure 4

Duration of Ifngr1 silencing by siRNA Ifngr1 1641 in mouse tail skin

(A) Ifngr1 mRNA levels over four weeks post injection (n = 3 per group, single dose at 20 mg/kg); mRNA levels were measured using QuantiGene 2.0 assays, Ifngr1 expression was normalized to the housekeeping gene Ppib; data were represented as percent of NTC control (mean ± standard deviation) and analyzed by unpaired t test (∗p < 0.05; ns, not significant). (B) IFNGR1 protein levels were determined by the relative MFI of anti-mouse IFNGR1 staining in CD45+ (hematopoietic cell marker) cell population; MFI values were subtracted from Ifngr1−/− background staining, and normalized to NTC control (mean ± standard deviation, unpaired t test, ∗∗p < 0.01, ∗∗∗p < 0.001). (C) Representative flow cytometry panels of CD45+ cells and IFNGR1 staining at week 2 post-injection. (D) Histograms of graph (C) for IFNGR1 staining in CD45+ cells.