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. 2022 May 6;30(8):2693–2708. doi: 10.1016/j.ymthe.2022.05.002

Figure 3.

Figure 3

Efficient knockdown of BCL11A and ZNF410 by double shmiR vector in erythrocytes differentiated in vitro from β-thalassemia patient gene-marked CD34+ HSPCs

(A) BCL11A and ZNF410 mRNA expression was measured by qRT-PCR with GAPDH as control on day 11 of differentiation. (B) Induction of globin mRNA was determined on day 18 of differentiation by qRT-PCR. (C) Hemoglobin of cell lysates was measured by HPLC on day 18 of differentiation. (D) Cell size by relative forward scatter intensity of enucleated erythroid cells, normalized to healthy donor. Data represent mean ± SD; n = 3; ∗p < 0.05; ∗∗p < 0.01;.