Figure 5.

Correction of in vivo hematologic parameters in Berkeley SCD mouse model

Mice were bled at weeks 4, 8, 12, and 16 and peripheral blood (PB) was analyzed. (A) PB engraftment in transplanted mice. (B) Red blood cell (RBC) count, (C) percentage of hematocrit (HCT), (D) hemoglobin (HGB) level, and (E) reticulocyte counts. (F) The percentage of sickled RBCs in PB after MBS treatment was quantitated. (G) Percentage of total β-like globin as γ-globin mRNA expression of erythroid cells in PB determined by qRT-PCR. (H) Representative fluorescence-activated cell sorting (FACS) plots of PB cells stained for the erythroid differentiation markers CD71 and Ter119 at week 16. (I) Summary of the percentages of CD71⁺ Ter119⁺ high erythroid precursor cell population. Data represent mean ± SD. Symbols indicate mice transplanted with different shmiR vectors or nontransduced cells; each data point represents an individual mouse. ns, not significant; ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.