Figure 6.

HbF expression in vivo in BM cells from Berkeley SCD mouse model

Whole BM was taken from each mouse at week 16 and analyzed. (A) BM engraftment in transplanted mice. (B) VCN in BM was determined. (C) Percentages of γ-globin mRNA expression of erythroid cells in BM determined by qRT-PCR. (D) Correlation of γ-globin expression assessed by qRT-PCR in erythroid cells of BM versus sickled cells in PB after MBS treatment; correlation coefficient (r²) is shown for all of the data. (E) Percentage of HbF⁺ cells by flow cytometry analyses in human CD235a⁺ erythroblasts isolated from BM. (F) Spleen weights at week 16. Data represent mean ± SD. Each data point represents an individual mouse. ns, not significant; ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.