TABLE 2.
Zinc contents of the wild-type and mutant ColH enzymes
Enzyme | Zinc content (mol/mol of protein)a |
---|---|
Wild type | 0.92 |
H415F | 0.10 |
H415A | 0.45 |
E416D | 0.88 |
E416Q | 1.15 |
H419R | 0.17 |
N439A | 0.92 |
E446Q | 0.94 |
E446A | 0.79 |
E446D | 0.89 |
E447Q | 0.39 |
E447A | 0.32 |
E447D | 0.18 |
E451Q | 0.64 |
E451A | 0.80 |
E451D | 0.97 |
Values are averages for duplicate determinations with <10% variation.