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. 2022 Jul 29;13:928593. doi: 10.3389/fimmu.2022.928593

Figure 2.

Figure 2

Identification of IRF5 as a target of miR-144/451. Nucleotide sequence alignments showing complementarity between the 3′-UTRs of IRF5 mRNAs and miR-144 (A). Native and mutant version 3′-UTRs of IRF5 mRNAs were inserted into the pGL3-BS vector (B), and statistic bars represent the Firefly/Renilla luciferase activity detected in 293T cells (C). Expression of miR-144 (D), miR-451 (E) and IRF-5 Mrna (F) was detected in 293T cells after 24 h lentiviruses infection. Expression of miR-144 (G), miR-451 (H) and IRF-5 mRNA (I) were detected in fresh isolated DCs from the spleen of WT or miR-144/451 KO mice. Expression of miR-144 (J), miR-451 (K) and IRF-5 mRNA (L) were detected in DCs 24 h after lentiviruses infection (n = 3). Expression of IRF-5 protein was assayed in DCs 48 h after lentiviruses infection (M), and bars represent the ratio of IRF-5/β-actin (N). Ns. no significance, *p<0.05, **p<0.01, ***p<0.001.