Figure 4.

Knockdown of IRF-5 inhibits activities of DCs. Expression of IRF-5 mRNA was detected in DCs after 24 h lentiviruses infection (A). IRF-5 protein level was measured in DCs after 48 h lentiviruses infection (B), and statistic bars represent the ratio of IRF-5/β-actin (C) n = 5). Expression of CD80 (D, E) and TNF-α (F, G) on splenic DCs after lentiviruses infection. The statistical graph represents the mean fluorescence intensity gated on CD11b⁺CD11c⁺ cells. Ns. no significance, **p<0.01, ***p<0.001.