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. 2022 Jul 29;16:881175. doi: 10.3389/fncel.2022.881175

FIGURE 5.

FIGURE 5

Overexpression of miR-154-5p impairs EPC functions and inhibits angiogenesis following EPC transplantation in VaD rats. After 7 days culture, BM-EPCs were transfected with normal control (NC) scrambled RNA or miR-154-5p mimics, and EPC functions were then evaluated using tube formation assay (A,B), migration ability (C,D), and adhesion capacity (E,F). (A): 50×: scale bar, 100 μm; (C,E): 200×: scale bar, 100 μm. VaD rats were subjected to a single injection of saline or BrdU-labeled EPCs, which were transfected with NC RNA and miR-154-5p mimics. Seven days later, brain samples were harvested and stained with CD31 (green) and BrdU (red). (G) Representative images in the cortex region of VaD rats. 200×: scale bar, 100 μm [(A–F): n = 6; (G): n = 3]. *p < 0.05. Data are shown as mean ± SD. BrdU, 5-bromo-2′-deoxyuridine; EPC, endothelial progenitor cell; VaD, vascular dementia.