FIG. 4.

Detection of flagellin proteins produced by cells isolated from media that influence the expression of swimming and swarming motility. Proteins were detected by immunoblot analysis with an anti-flagellin-specific monoclonal antibody. Each lane contains cells at 0.25 OD₆₀₀ units per ml, isolated from 0.6% agar plates incubated for 18 h at 26°C. Lanes: TYE, T medium supplemented with yeast extract; LB, LB medium; TN, T medium supplemented with 1% NaCl; TGN, TG medium supplemented with 1% NaCl; TG, TG medium; T, T medium. The bracket at the left of the figure indicates the location of the detected flagellin.