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. 2022 Jul 29;13:909252. doi: 10.3389/fmicb.2022.909252

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Copyright © 2022 Fan, Wang, Jiang, Gao, Niu, Zhang, Huang, Bao, Liu, Wang, Gao, Li, Cui, Pan, Liu, Zhang, Wang and Qi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of antigen epitopes with MAbs 7D4 and 2-5C-6F. (A) Schematic diagrams of all peptides (not drawn to scale) used in the peptide-scanning method. (a) Three overlapping peptides spanning the VP2 aa sequences. (b) Identification of the antigen epitope with MAb 7D4. (c) Identification of the antigen epitope with the MAb 2-5C-6F. (B) Recognition of the minimal epitope with 2-5C-6F by Western blotting. Lane 1 corresponds to the epitope of aa 317-336; 2, aa 318-336; 3, aa 319-336, 4 corresponds to aa 317-336; 5, aa 317-335; M, marker. (C) Recognition of the minimal epitope with MAb 7D4 by Western blotting. 1, aa 183-196; 2, aa 184-196; 3, aa 185-196; 4, aa 183-193, 5, aa 183-192, 6, aa 183-191; 7, aa 183-190; M, marker.