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. 2022 Jul 29;13:909252. doi: 10.3389/fmicb.2022.909252

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Copyright © 2022 Fan, Wang, Jiang, Gao, Niu, Zhang, Huang, Bao, Liu, Wang, Gao, Li, Cui, Pan, Liu, Zhang, Wang and Qi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Antigen recognition detection of the wild and mutated types of VP2 in DF1 cells directed by MAbs 7D4 and 2-5C-6F. (A) VP2 of the SHG19 strain (SHG19VP2) and its mutated types (SHG19VP2-D318G, SHG19VP2-E323D, and SHG19VP2-D318G/E323D). (B) VP2 of the Gx strain (GxVP2) and its mutated types (GxVP2-G318D, GxVP2-D323E, and GxVP2- G318D/ D323E). (C) Quantitative analysis of fluorescence of (A). (D) Quantitative analysis of fluorescence of (B). The mean fluorescence intensity and standard deviations (error bars) from three independent samples are shown. * represents P < 0.05, ** represents P < 0.01, *** represents P < 0.001, **** represents P < 0.0001.