Nav1.8 knockdown block rosacea-like development. A, Schematic diagram of intradermal injection of Nav1.8 for continuous 3 days before intradermal injection of LL37 in mice. B, The mRNA levels of Nav1.8 by siRNA in LL37-induced mice. C, The back skins of NC (negative control siRNA) and Nav1.8 knockdown (Nav1.8 siRNA) mice injected with LL37 or PBS. D, The reduced redness score, area of erythema and skin thickness of skin tissues in PBS/LL37-induced mice. Data represents the mean ± SEM. **p < 0.01, ***p < 0.001.2-way ANOVA test was used. E, HE staining of lesional skin of NC and Nav1.8 knockdown mice injected with LL37 or PBS. F, The mRNA levels of IL1β, IL-6, TLR2 and MMP-9 in mice skin lesion. Data represents the mean ± SEM. **p < 0.01, ***p < 0.001.2-way ANOVA test was used. G, The CD4+ T cells infiltration in mice skin lesion. H, The CD31+ microvascular in skin visualized by immunofluorescence. (n = 6 for each group).