SDS-PAGE of purified proteins. Lanes: 1, Eap purified from Eap-Sepharose; 2, molecular mass markers (14, 20, 30, 43, 67, and 94 kDa from bottom); 3, Eap purified by FPLC; 4, coagulase purified by FPLC; 5, Efb purified by FPLC; 6, recombinant His-Clf.