FIG. 5.

(A) Binding of ¹²⁵I-labeled Eap to S. aureus Newman. To a constant number of bacterial cells (10⁶ CFU) were added different amounts of radiolabeled Eap. Binding was allowed for 1 h, the cells were washed, and bound radioactivity was determined. (B) Competitive inhibition of Eap binding to S. aureus. Cold Eap (closed squares) or coagulase (open squares) was added at the indicated concentrations to cells of S. aureus (10⁶ CFU), to which was simultaneously added ¹²⁵I-labeled Eap (1 μg). Binding of Eap was determined as described for Fig. 4.