TABLE 1.

Amount of Eap isolated from 4- and 24-h cultures from the supernatant and from LiCl extracts of the bacteria^a

Culture	Fraction	Yield (mg/liter)	%
4 h	LiCl extractb	0.42	29
	Supernatantb	1.05	71
24 h	LiCl extractb	3.75	37
	Supernatantb	6.47	63
Protein A	LiCl extractc	5	>98
	Supernatantc	<0.1	<2

^aProtein A was used as a control for protein extraction. 

^bEap was purified by Fg affinity chromatography followed by ion exchange. 

^cProtein A was purified by immunoglobulin G affinity chromatography.